Dr. Anita Krisko

Dr. Anita Krisko2020-03-06T12:31:47+00:00

Macromolecular homeostasis in aging

Aging is a natural phenomenon that occurs despite complex pathways of maintenance and repair. As chronological age of an organism increases, a number of errors accumulate at different levels of biological organization. The tendency of errors to accumulate and cause downstream problems in maintenance of cellular homeostasis is met by numerous protection and repair mechanisms.

Cellular proteostasis is maintained by a network of molecular chaperones, protein degradation machineries, and stress-response pathways, whose coordinated action senses and counteracts protein misfolding. The efficiency of proteostasis may decline, with well described consequences, especially in the context of numerous diseases and aging. Despite the indisputable importance of cellular proteostasis on multiple levels, the relationships between proteostasis and other cellular pathways remain poorly understood.

Our group seeks to understand the interplay between RNA and protein homeostasis and the cellular metabolic activity, as well as the role it has in cellular quality control. We focus on elucidation of cellular and molecular mechanisms underlying the ribostasis-proteostasis-metabolism crosstalk within and across cellular compartments. We aim to obtain an integrated picture of the effects that the quality of macromolecular folding environment is able to yield on cellular metabolism, thus contributing to understanding of the implications of this multifaceted network in aging and disease. Understanding this crosstalk may reveal novel insights into basic cellular functioning, and shed new light on the process of aging, and neurodegenerative diseases.

Funding

Education and Positions

  • 2019 – group leader (Heisenberg programme), University Medical Center Goettingen

  • 2014 – 2019, assistant professor, Faculty of Science, University of Split.

  • 2012 – 2019, group leader, Mediterranean Institute for Life Sciences, Split

  • 2007 – 2012, senior postdoctoral fellow, INSERM U1001, Faculté de Medecine Paris 5

  • 2006 – 2007, postdoctoral fellow, Equipe de Bioinformatique Genomique et Moleculaire, Université Denis Diderot – Paris 7

  • 2002 – 2005, PhD student, Molecular biophysics, Rudjer Boskovic Institute, Zagreb, Croatia

  • 2001, BSc (equivalent of Master), Molecular biology, Department of Physical Chemistry, Rudjer Boskovic Institute & Department of Biology, Faculty of Science, University of Zagreb, Croatia.

Teaching activities

  • 2014 – assistant professor, Faculty of Natural Sciences and Mathematics of University of Split, courses: Bioinformatics; Research in Bioinformatics

  • 2000 – 2005, teaching assistant in Biophysics, Faculty of Science, Zagreb, Croatia

Selected Grants, Honors and Awards

  • 2019 – Heisenberg Grant, German Research Foundation (DFG)

  • 2017 – Member, Research Network “Proteostasis” COST-BM1307

  • 2016 – Member, Research Network “GENiE” COST-BM1408

  • 2016 – 2017 ,  Guest Editor, Frontiers in Cell and Developmental Biology, research topic: Mitochondrial Communication in Physiology, Disease and Aging

  • 2015, Center of Excellence in Science and Technology Research Grant (external member)
  • 2009, National Science Award for Young Researchers from the Parliament of the Republic of Croatia.
  • 2009, City of Varazdin honorary award.
  • 2006, Award of the Rotary Club Varazdin, Croatia, Best PhD in 2005.
  • 2006, UNESCO-L’Oreal prize ‘For Women in Science’
  • 2001, Chancellor’s Award at the University of Zagreb

Organization of meetings and science popularization events

  • 2017, Organizer, COST-BM1408 Meeting ‘Proteostasis in Aging and Disease’, Split, Croatia

  • 2013, participant, Researchers’ Night, Split, Croatia

  • 2012, participant, Science Festival, Split, Croatia

  • 2008, Organizer, International Summer School ‘Life in Extreme Conditions’, Split, Croatia
  • 2006 , Organizer, International Summer School ‘Structure and Evolution – From Bench to Terminal’, Split, Croatia
  • 2006-2010, Co-organizer, series of exhibitions of scientific photography ‘Science in Pictures: Hidden Reality’

Selected lectures

  • 2019, FEMS International Summer School for Postdocs

  • 2019, Conference ‘Interdisciplinary Endeavour in Technology at Nanoscale’

  • 2018, International School, 1st Autumn School of Proteostasis

  • 2018, Goettingen Organelle Network Symposium

  • 2017, Symposium of the RNA Club Bordeaux

  • 2017, International Conference ‘Advances in Biomedical Research’

  • 2012, NATO Advanced Research Workshop ‘Multidisciplinary Endeavour in Nanobiology, Nanoscience and Environment’

Editorials

  • Cross-organelle communication at the core of longevity
    Raimundo N, Kriško A.
    Aging (US) 2018; 10: 15-16.

  • Mitochondrial communication in Physiology, Disease and Aging.
    Raimundo N, Kriško A.
    Front Cell Dev Biol. 2019, 7: 54.

Selected recent publications

  • Protein damage, ageing and age-related diseases.
    Krisko A, Radman M.
    Open Biol. 2019 Mar 29;9(3):180249. doi: 10.1098/rsob.180249.
    PubMed

  • Heat-induced longevity in budding yeast requires respiratory metabolism and glutathione recycling.
    Musa M, Perić M, Bou Dib P, Sobočanec S, Šarić A, Lovrić A, Rudan M, Nikolić A, Milosević I, Vlahoviček K, Raimundo N, Kriško A.
    Aging (Albany NY). 2018 Sep 17;10(9):2407-2427. doi: 10.18632/aging.101560.
    PubMed

  • Normal mitochondrial function in Saccharomyces cerevisiae requires inefficient splicing.
    Rudan M, Bou Dib P, Musa M, Kanunnikau M, Sobocanec S, Rueda D, Warnecke T*, Kriško A*.
    eLife 2018; 7:e35330.
    *equal contribution
    PubMed

  • TORC1-mediated sensing of chaperone activity alters glucose metabolism and extends lifespan.
    Perić M, Lovrić A, Šarić A, Musa M, Bou Dib P, Rudan M, Nikolić A, Sobočanec S, Mikecin AM, Dennerlein S, Milošević I, Vlahoviček K, Raimundo N, Kriško A.
    Aging Cell. 2017 Oct;16(5):994-1005. doi: 10.1111/acel.12623. Epub 2017 Jun 14.
    PubMed

  • Crosstalk between cellular compartments protects against proteotoxicity and extends lifespan.
    Perić M, Bou Dib P, Dennerlein S, Musa M, Rudan M, Lovrić A, Nikolić A, Šarić A, Sobočanec S, Mačak Ž, Raimundo N, Kriško A.
    Sci Rep. 2016 Jun 27;6:28751. doi: 10.1038/srep28751.
    PubMed

  • Decreasing translation error rate in Escherichia coli increases protein function.
    Musa M, Radman M, Krisko A.
    BMC Biotechnol. 2016 Mar 11;16:28. doi: 10.1186/s12896-016-0259-8.
    PubMed

  • RNA chaperones buffer deleterious mutations in E. coli.
    Rudan M, Schneider D, Warnecke T, Krisko A.
    Elife. 2015 Mar 25;4. doi: 10.7554/eLife.04745.
    PubMed

  • Signatures of conformational stability and oxidation resistance in proteomes of pathogenic bacteria.
    Vidovic A, Supek F, Nikolic A, Krisko A.
    Cell Rep. 2014 Jun 12;7(5):1393-1400. doi: 10.1016/j.celrep.2014.04.057. Epub 2014 May 29.
    PubMed

  • Inferring gene function from evolutionary change in signatures of translation efficiency.
    Krisko A, Copic T, Gabaldón T, Lehner B, Supek F.
    Genome Biol. 2014 Mar 3;15(3):R44. doi: 10.1186/gb-2014-15-3-r44.
    PubMed

Selected publications

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